Subcloning by restriction digestion is a commonly used laboratory technique. Our technique can be used to easily move any DNA fragment from one vector to another, as long as it has been restricted by restriction sites that are also present in the same orientation on the target vector.

Design

Creative Enzymes has access to many DNA analysis tools that allow you to identify which restriction sites are present in a given sequence. When choosing a restriction endonuclease, you will need to select one that has

• Side cut your blade, but not inside your blade
• Cuts at the desired location in your recipient plasmid, but not elsewhere in the plasmid
• Conform to the frame of the tag or fusion protein in the recipient plasmid

  Creative Enzymes is a company that provides professional and comprehensive plasmid cloning service by restriction enzymes service. We have years of experience to meet your specific project needs in using enzyme research to add value to your research projects. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.

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